均匀设计与正交设计在金莲花提取工艺筛选研究中的比较应用

The Comparative Studies on the Extracting Techniques of Jinlianhua by Even-design and Orthogonal-design Methods

摘要:

应用均匀设计与正交设计两种方法对金莲花提取工艺进行研究,考察不同提取条件下的提取率及样品中总黄酮的含量,并用同一标准评价两种设计方法。结果:正交设计认为影响金莲花提取率及总黄酮含量的最主要因素均是提取时的乙醇浓度,两者的最佳乙醇浓度均应是65%;均匀设计认为对提取率而言最大的影响因素也是乙醇浓度,最佳乙醇浓度应是60%,对总黄酮含量而言最大的影响因素也是乙醇浓度,最佳乙醇浓度为70%。表明:正交设计与均匀设计试验所得的结果相近,结论基本一致。

Abstract:

Objective:To compare the extracting methods of Jinlianhua evaluated by even-designed and orthogonal-designed methods.Results:The main factors to extract Jinlianhua evaluated by extracted yield and total flavones were 65% alcohol with orthogonal design method,and 65% and 70% alcohol with even-design method.Conclusion:The similar results were obtained by those two methods.