大黄中总大黄素成分含量测定条件优选

Determination Method of Anthraquinonoids in Radix et Rhizoma Rhei

摘要:

目的:优选大黄中总大黄素成分含量测定条件。方法:采用均匀设计法,HPLC测定其含量,对影响总大黄素含量的主要因素:水解时间、酸的浓度、酸用量、氯仿提取次数及氯仿用量5个因素进行考察,结果利用UROS系统软件回归处理,得出优化条件:水解时间9min;氯仿用量70ml;提取次数2次;H₂SO₄用量10ml;H₂SO₄浓度1mol/L经验证试验,重复性好,结果可靠。

Abstract:

Objective:To select the optimum extracted method of anthraquinonoids from radix et rhizoma Rhei by RP-HPLC.Methods:The main factors effecting the total anthraquinonoids,such as hydrolysis time,concentration and volume of acid,extraction times and volume of chloroform were examined by uniform design mthod.The data were calculated by UROS system.Results:The optimum extracted conditions:hydrolysis with 1mol/L H₂SO₄ 10ml for 9min,extracted by 70ml chloroform for two times.Conclusion:This method was validated to show reliable and reproducible.