当归芍药散防治老年期痴呆的物质基础与作用机理研究Ⅰ——组方作用协同性与选择性研究

Study I on Substance Basis and Mechnaism of Action of Danggui Shaoyao San in the Prevention and Treatment of Senile Dementia-Cooperative and Selective Actions in the Prescription

摘要:

分别用东莨菪碱与反复缺血再灌注制备阿尔采末病(AD)与血管性痴呆(VD)模型,考察当归芍药散(DSS)防治老年期痴呆的配伍机理。避暗法与跳台法检测发现,AD组较正常组潜伏期缩短(P<0.01),错误次数增多(P<0.05～0.01);VD组较假手术组潜伏期缩短(P<0.01),错误次数增多(P<0.01),DSS中活血补血药组(DCS)与健脾利湿药组(FBZ)可协同延长AD小鼠潜伏期,减少错误次数(P<0.05～0.01);对VD小鼠,仅FBZ可延长潜伏期(P<0.05),减少错误次数(P<0.01),提示FBZ较DCS可能具有更大的防治意义。

Abstract:

Applying scopolamine and ischemia-reperfustion to establish murine models for Alzheimer's disease(AD) and vascular dementia(VD),respectively,might help explicate the formula mechanism of Danggui Shaoyao San(DSS).In comparison with the normal group,step-through latency taken by mice injured with scopolamine got shortened(P<0.01),step-through and step-down number of errors increased (P<0.05～0.01);Both drugs used to promote blood circulation and nourish the blood (DCS) and drugs used to nourish the spleen and disperse the pathogenic damp (FBZ) in DSS at low dosage,made step-through latency longer (P<0.05),decreased obviously step-through and step-down number of errors (P<0.05～0.01).If DCS and FBZ were in combination with each other,their cooperative melioration would be furthered;Compared with the sham operational group,step-through latency taken by ICR mice injured with ischemia-reperfusion got shortened(P<0.01),step-through number of errors increased (P<0.01),However,it was FBZ other than DCS that could at high dosage elongate step-through latency (P<0.05),decrease obviously step-through number of errors (P<0.01),antagonize selectively the memory impairment of ICR mice induced by ischemia-reperfusion,those discovery maybe indicated that to nourish the spleen and disperse the pathogenic damp played a more important role in the prevention and treatment of senile dementia than to promote blood circulation and nourish the blood.