川芎嗪预处理对清醒大鼠心肌缺血再灌注损伤的保护作用

Ligustrazini preconditioning protection on myocardial ischemia and reperfussion injury in the conscious rat

摘要:

目的:用心肌缺血预处理的整体动物模型,观察川芎嗪预处理对清醒大鼠心肌缺血灌注损伤所致心律失常、心肌梗塞面积、乳酸脱氢酶和磷酸肌酸激酶活性的保护作用。方法:先将实验动物麻醉,在人工呼吸状态下,开胸,左主动脉下穿线。然后,缝合胸腔。术后第6d,进行实验。单纯缺血再灌注组:结扎冠脉30min,其后再灌120min;缺血预处理组:冠脉结扎5min,再灌5min;然后,再次结扎冠脉30min,其后再灌120min;药物组:川芎嗪20mg·kg^-1和40mg·kg^-1分别连续静脉给药5min;5min后,结扎冠脉30min,其后再灌120min;结果:川芎嗪降低缺血和再灌时间VF和VT的发生,其发生率为375%;使心肌梗塞面积缩小,乳酸脱氢酶和磷酸肌酸激酶的释放减少;上述指标与单纯缺血再灌注组相比,均有显著性差异。其结果与缺血预处理(冠状结扎5min,再灌5min)对心肌缺血再灌注损伤产生的保护作用一致。结论:川芎嗪通过预处理途径对清醒大鼠心肌缺血再灌注损伤所致心律失常、心肌梗塞面积、乳酸脱氢酶和磷酸肌酸激酶活性产生保护作用。

Abstract:

Lgustrazini(20mg·kg^-1 and 40mg·kg^-1) was injected to the conscious rat for 5min,then followed by ischemia 30min/reperfussion 120min,the incidence of VT and VF and mean duration of VT plus VF were decreased with a delayed onset of VT plus VF.In addition,the rise of plasma level of LDH,CPK and the infarct size was attenuated.The result indicated that pretreatment of ligustrazini might mimic IP with cardiacprotection on ischmia/reperfussion.