脑络通对大鼠脑缺血再灌注损伤血清与脑组织NO含量及NOS活性影响的分期观察

Periodic Observation for NO Level and NOS Activity in Serum and Brain Tissue of Rats Damaged by Cerberal lschemic Reperfusion with Naoluotong

摘要:

目的:探讨中药脑络通改善脑缺血损伤的作用机制。方法:多因素复合制作气虚血瘀证大鼠脑缺血动物模型,采用化学比色法测定血清与脑组织匀浆一氧化氮(NO)浓度及脑组织匀浆一氧化氮合酶(NOS)活性。结果:预防治疗组可以显著升高缺血急性期病理性降低的血清NO含量,降低缺血急性期病理性升高的脑匀浆NO含量、NOS活性。结论:具有益气活血功效的中药脑络通对于缺血性中风具有预防和治疗作用。

Abstract:

It was studied that the mechanisms of Naoluotong on preventing and treating cerebral ischemicreperfusion damage rats.The cerebral ischemic model with Qi-dificiency and blood stasis syndrome was established to observe the level of nitric oxide (NO) in serum and brain tissue,and the activity of nitric oxide synthase (NOS) of brain tissue through chemical colorimetric method.The results show that Naoluotong can remarkably raised the level of NO in serum,and decreased the level of NO and the activity of NOS in brain tissue.