茵陈有效成分对四氯化碳损伤的原代培养大鼠肝细胞的作用

Effects of Active Components of Herba Artemisiae Capillaris on Rat primary Culture Hepatocytes Injured by CCl4

摘要:

目的:研究茵陈有效成分对四氯化碳损伤的原代培养肝细胞活力及ALT含量的影响。方法:采用四甲基偶氮唑盐(MTT)比色法测定肝细胞活力,赖氏法测定培养液中丙氨酸氨基转移酶(ALT)含量。结果:菌陈有效成分Ⅱ、Ⅳ、Ⅴ、Ⅵ都能使四氯化碳损伤的肝细胞活力明显提高;培养液中ALT含量显著降低。结论:菌陈有效成分Ⅱ、Ⅳ、Ⅴ、Ⅵ对四氯化碳损伤的原代培养大鼠肝细胞都有保护作用

Abstract:

It was studied on the active components of Herbal Antimissile Capillaries on the primary culture rat hepatocytes injured by CCl₄ and the effects on contents of ALT.MTT colorimetric determination was applied to examine the activity of hepatocytes,and by another method,the content of ALT on the culture liquid was determined.The results showed active component Ⅱ,Ⅳ,Ⅴ,Ⅵ of the herb could obviously increase the activity of hepatocytes injured by CCl₄ and decrease the ALT content in culture liquid.