复方柴胡汤大孔吸附树脂分离精制工艺的研究

Studies on Pureparation Techniques of Compound Caihutang

摘要:

以柴胡皂苷A,芍药苷,橙皮苷,甘草酸四种指标成分的含量为指标,考察复方柴胡汤大孔吸附树脂精制工艺。结果选用1倍(生药)量S型大孔树脂,以1倍量水洗,4倍(生药量)70%乙醇洗脱树脂为最佳工艺,大孔树脂反复使用40次,干膏率、橙皮苷及芍药苷含量均无下降趋势。

Abstract:

The purification conditions by absorption resins were optimized by content determination of saikosaponin A,paeoniforin,hesperidin and glycyrrhizic acid.S type synthetic absorption resin was selected.The optimal purification conditions were:specific input sample amount(ratio of weight of medical raw material to volume of resin,W/V)was one,ltime of water to elute the resin and collecting 4 times of 70% ethanol(V/W) as elution solvent.The yield and contents of paeoniforin and hesperidin of dried extract,purified by the absorption resin used repeatedly for 40 times,was not decreased compared with the new resin.