养阴益气活血冲剂对培养人脐静脉内皮细胞抗凝和纤溶等功能的影响

The influence of Yin-nourishing Qi-tonifying and Blood-moving Granules on the Anticoagulation and Fabrinolysis by Cultivating endotheliocyte of human umbilical vein

摘要:

目的:研究养阴与益气活血方药对培养人脐静脉内皮细胞抗凝和纤溶等功能的影响。方法:采用血清药理学方法观察养阴益气活血方药对培养人脐静脉内皮细胞抗凝、纤溶及抗自由基损伤的作用。结果:养阴益气活血方药具有显著增高前列环素(6-keto-PGF1α)含量;提高组织型纤溶酶原激活物(t-PA)的活性,抑制组织型纤溶酶原激活抑制物(PAI)活性的作用;提高超氧化物歧化酶(SOD)活力,降低丙二醛(MDA)含量的作用;降低ET含量,提高NO含量等作用。结论:养阴与益气、活血方药相伍在抗栓、溶栓、抗自由基损伤等方面具有协同作用,认为养阴生津方药是治疗血瘀证的重要法则。

Abstract:

To studying the influence of Yin-nourishing Qi-tonifying and blood-moving Granules on the anticoagulation and fabrinolysis by cultivating endotheliocyte of human umbilical vein,obersevation of Yin-nourishing Qi-tonifying and blood-moving recip to the anticoagulation,and fabrinolysis and anti-injury of free radical of cultivating endotheliocyte of human umbilical vein by using the method of serology pharmacology. Yin-nourishing Qi-tonifying and blood-moving recip could obviously increase the content of (6-keto-PGF1α);It could promote the activity of t-PA and inhibit the activity of PAI;It could promote the activity of SOD and decrees the content of MDA;Yin-nourishing recipe could decrease the content of ET and increase the content of NO. The function of the anticoagulation,fabrinolysis and anti-injury of free radical by Yin-nourishing Qi-tonifying and blood-moving recip was much superior than Qi-tonifying and blood-moving recip,with blood-moving recip.