补肾益智方拆方含药血清保护Aβ25-35损伤NG108-15细胞的研究

Protective Effect of the BSYZF-Contained Serum and its Subgroups on Aβ25-35 Induced NG108-15 Cells Injure

摘要:

目的:研究中药补肾益智方拆方亚组的含药血清对AD细胞模型生长、分化等方面的影响,探讨该方药物的配伍规律。方法:将补肾益智方拆分为补肾组、益气养血组和去冰片组(即补肾组+益气养血组)等3个亚组,连同原方组共4组对大鼠灌胃给药分离药物血清。在含各组血清的培养液中,加入Aβ25-35,观察NG108-15细胞的生长状态、细胞增殖数、存活率,突起率及突起平均长度。结果:与AD细胞模型组相比,补肾益智方及各亚组含药血清都能在一定程度上抑制Aβ25-35对细胞的损伤作用,但各组情况有所不同。各组含药血清对细胞的保护作用由强至弱依次是去冰片组>补肾益智方组>补肾组>益气养血组。结论:补肾药配伍益气养血药可增强对Aβ25-35损伤NG108-15细胞的保护作用,其中以补肾药的作用为主,方中冰片的功效有待进一步研究。

Abstract:

To study the effects of the BuShenYiZiFang-contained serum (BSYZF) and its subgroups on the differentiation and growth of NG108-15 cells injured with Aβ25-35,and to investigate compatible effects of BSYZF.BSYZF was divided into three subgroups:BUSHEN group,YIQIYAANGXUE group,QUBINGPIAN group (BUSHEN plus YIQYANGXUE,i.e.deleted borneol BSYXF).Four groups (BSYXF and its three subgroups) serum containing correspondent drugs were collected from rats,respectively,and used to culture cells.NG108-15 cells were cultured with Aβ25-35 for establishing cell model of Alzheimer's disease (AD).The cell morphology,cell viability and proliferation,and neurite outgrowth were observed.The results showed that all the herbage-contained serum could protect NG108-15 cells from Aβ25-35 neurotoxicity and the QUBINGPIAN was likely to have the best protective effect.The protective effects of the four groups were in the order:QUBINGPIAN>BSYZF>BUSHEN>YIQAIYANGXUE.