开郁清胃颗粒对链脲佐菌素诱发糖尿病大鼠降糖机理的实验研究

Experimental Study on Therapeutic Mechanism of Kaiyu Qingwei Granules in Reducing Blood Sugar

摘要:

目的:探讨对STZ糖尿病大鼠模型降糖作用的机制。方法:用血糖仪检测了开郁清胃颗粒对STZ糖尿病大鼠血糖的影响;用放免分析法测定了中药治疗后胰岛素、胰高血糖素、皮质醇、胃动素、胰岛素样生长因子-1(IGF-1)含量的变化;用左旋木糖试验测定了药物对小肠吸收功能的影响。结果:开郁清胃颗粒能降低糖尿病模型大鼠的血糖、皮质醇水平;升高胃动素、胰岛素样生长因子-1水平;对体重、胰岛素、胰高血糖素和小肠吸收功能无明显影响。结论:开郁清胃颗粒能降低糖尿病模型大鼠的血糖,其降糖机理不同于二甲双胍抑制葡萄糖在小肠的吸收;也不同于磺脲类增加胰岛的分泌功能;而是升高IGF-1和胃动素水平,降低皮质醇水平。

Abstract:

To study the mechanism of Kaiyu Qingwei Granules in reducing blood sugar in STZ diabetes rats,blood sugar in STZ diabetes rats was determinated by one-touch Ⅱblood sugar instrument.The level variations of insulin,glucagons,cortisol,motilin,insulin-like growth factor-1(IGF-1) and the absorb function in small intestine were measured by radioimmunoassay (RIA) and dextroxylosetest,respectively.Results showed Kaiyu Qingeci Granules could reduce the level of blood sugar and cortisol in STZ diabetic rats,and increase the level of IGF-1 and motilin.It did not obvious effect on body weight,insulin,glucagons and the function of glucose absorb in small intestine.