斑秃生发颗粒质量标准的研究

The Quality Standard of Bantushengfa Granules

摘要:

目的:建立斑秃生发颗粒的质量标准。方法:用HPLC法对斑秃生发颗粒中主要成分2,3,5,4′-四羟基二苯乙烯-2-0-β-D葡萄糖苷进行含量测定,流动相为乙腈-3%冰乙酸水(12∶88),检测波长320nm。同时对制剂中的主要药味黄芪、何首乌、当归、羌活进行薄层鉴别。结果:平均加样回收率为99.33%,RSD=1.74%(n=6)。薄层图谱斑点清晰,阴形对照无干扰。结论:本方法可靠准确、重现性好,可有效控制制剂的质量。

Abstract:

Objective:To establish the quality of Bantushengfa granules.Methods:The content of main component,2,3,5,4′-tetrohyoxystilbene-2-O-β-D-glucoside,was determined by HPLC.The mobile phase consisted of acetontrile-3% acetic acid water solutoion(12∶88) and UV detection wavelength was 320nm.Result:The average recovery and RSD were 99.33% and 1.74%,respectively.Conclution:The method is accurate,reproducible and can be used for content of Bantushengfa granules.