HPLC法测定中药复方OPG片中橙皮苷的含量

Determination of Hesperidin in Compound OPG Tablet by HPLC

摘要:

目的:建立测定中药复方OPG片中橙皮苷含量的方法。方法:样品用甲醇超声提取30min,在C₁₈柱上以甲醇-水(41∶59)为流动相,在波长283nm处紫外检测。结果:橙皮苷含量测定的线性范围为0.05658～1.13μg,线性回归方程为Y=2637.54768X-7.43358,r=0.9999,平均回收率为104.94%,RSD为2.37%。结论:该法简便、准确,重现性好,可作为中药OPG片质量控制的方法。

Abstract:

Obective:To develop a HPLC method for determining Hesperidin in the Compound OPG Tablet.Method:The sample was sonicated for 30 min.The contents were determined by HPLC,with a C18 column a molile phare of methanol-water (41∶59) and detection wavelength at 283nm.Result:Hesperidin has a good linearity in the range of 0.05658～1.13μg,Y=2637.54768X-7.43358,r=0.9999,The average recovery is 104.94% and RSD=2.37%(n=5).Conclusion:The method is sensitive,reliable and speedy.It can used for quality control of Compound OPG Tablet.