扶正祛邪中药复方对猴艾滋病模型实验研究

Study on Effect of Fuzheng Quxie Chinese Drugs on Monkey of SIV Infection Moden

摘要:

扶正祛邪中药复方“艾克冲剂”对猴SIV感染模型(SAIDS)治疗作用。方法:利用SIVmac病毒液静脉接种恒河猴造成猴艾滋病实验模型,流式细胞仪测定感染及治疗动物外周血CD⁺₄细胞、CD⁺₈细胞数及CD⁺₄CD⁺₈比值,并以MTT法观察T、B细胞对有丝分裂原诱导分化增殖反应。结果:艾克冲剂能提高CD⁺₄细胞百分数及CD⁺₄CD⁺₈比值,并能促进T、B淋巴细胞增殖。结论:扶正祛邪中药艾克冲剂能够提高、改善SIVmac感染动物免疫功能。

Abstract:

Objective:To Investigate the effect of Lyke Granule on simian immunodeficiency Virus(SIV) infection model. SAIDS model were used. Using monoclonal antibody label on CD⁺₄ and CD⁺₈ cells in PBMC with direct immunofluorescence,the percentage was analyzed by flow cytometry and analyzed CD⁺₄/CD⁺₈ ratio. T and B cells proliferation induced by mitogens was defected before treatment and after treatment with Lyke Granule. Results indicate that Lyke Granule enhanced the proliferation of T and B cells induced by motogen,CD⁺₄ T cells were increased in PBMC and CD⁺₄/CD⁺₈ were changed.