高效液相色谱法测定复方川芎胶囊中川芎嗪的含量

Dtermination of Tetramethylpyrazine in Fufang Chuanxiong Capules by HPLC

摘要:

目的:建立复方川芎胶囊中川芎嗪的含量测定方法。方法:用80%甲醇溶液提取,以Nova-pakC₁₈色谱柱(3.9mm×300mm,4μm)为固定相,甲醇-水(50:50v/v)为流动相,检测波长为282nm。结果:川芎嗪在5～40μg·ml^-1范围内呈良好的线性关系,r=0.9996;平均回收率为99.0%,RSD=1.2%;日内精密度是RSD=0.6%。结论:方法能消除其它成分的干扰,准确可靠、重现性好,操作简便快速。

Abstract:

Objectivt:To establish a determining method for Tetramethylpyrazine(TMP)in Fufang Chuanxiong Capules by high-perfomance liquid chromatography.Methods:Tetramethylpyrazine was extracted from Fufang Chuanxiong Capules by 80% methanol.Nova-Pak C_{18 colum(3.9mm×300mm,4μm) was used as stationary phase,methanol-H 2O(50:50v/v)was used as mobile phase,the flow rate was 0.7mL·min-1 and the wavelength of detection was at 282nm.Results:The calibration curve was showed good linearity within the concentration range of 5～50 μg·mL^-1 r=0.9996,the average recovery was 99.0%,RSD=1.2%.Conclusion:The established method is accurate,precise and simple.}