三七复合有效成分对恒河猴细菌感染性子宫出血模型内膜组织一氧化氮及一氧化氮合酶的影响

Influence of Two Kinds of Active Components from Panax Notoginseng on NO and iNOS in Inflammatory Metrorrhagia Model of Macaca mulatta

摘要:

目的:探讨一氧化氮(NO)及诱导型一氧化氮合酶(iNOS)在炎性子宫出血中的作用及三七复合有效成分的影响。方法:选择月经正常雌性育龄恒河猴11只,随机分为中药组(三七复合有效成分)4只、模型组4只及正常对照组3只。应用化学比色法检测三组子宫内膜中一氧化氮及一氧化氮合酶的活性,用免疫组化法检测三组子宫内膜中一氧化氮合酶(iNOS)蛋白的表达水平。结果:中药组与模型组比较,子宫内膜NO、iNOS的活性及iNOS蛋白表达水平明显降低,差异有显著性,P0.01;结论:NO可能参与了炎性子宫出血的发病机制,三七减少炎性子宫出血的机制可能与调节NO、iNOS的活性及iNOS蛋白表达水平有关。

Abstract:

Objective:To explore the influence of two kinds of active components from Panax notoginseng on NO and iNOS on inflammatory Metrorrhagia model of Macaca multatta. Methods:Eleven normal menorrhea adult Rhesus monkeys were choosed as research object,and were divided into model group (four),TCM group(four) and Control group(three) at random.Colorimetric method were performed to estimate the activity of NO and iNOS in of model group,TCM group and normal control group,the immunohistochemical S-p were performed to estimate the express level of iNOS in the endometrium of the three groups.Result:Compared to the model group,the activity of No and iNOS and the express level of iNOS in the endometrium of TCM gorup was obviously weaker,which are similar to that of the normal group.Conclusion:NO may be involred in the pathogenesis of inflammatory Metrorrhagia.Two kinds of active components from Panax notoginseng reduce inflammatory Metrorrhagia by regulating the activities of NO and iNOS and the express level of iNOS in the endometrium.