骨碎补总黄酮胶囊对实验性骨质疏松症和镇痛作用的影响

Effects of Capsule Assemble Flavones of Fortune's Drynaria Rhizome on the Experimental Osteoporosis in Rat and Analgesia Action in Mouse

摘要:

目的:应用维甲酸所致大鼠的实验性骨质疏松模型,观察骨碎补总黄酮对动物骨密度的作用及对血钙、血磷水平的影响。方法:维甲酸(70mg·Kg^-1·d^-1)给大鼠灌胃14d,可导致大鼠的实验性骨质疏松,然后观察药物对动物骨密度的影响;化学刺激疼痛试验采用小鼠腹腔注射醋酸所致小鼠扭体现象,观察各组动物的典型扭体发生次数;热传导刺激试验采用热板法,观察该药不同时间点的镇痛作用。结果:骨碎补总黄酮三个剂量组均可明显提高大鼠骨密度,提高大鼠血钙水平,和模型组比较有显著差异(P<0.05);同时骨碎补总黄酮胶囊对化学刺激(醋酸)有抑制作用,大剂量组和模型组比较有明显差异(P<0.05);对热传导刺激引起的拟痛反应也有明显的拮抗作用,和模型组比较有显著差异(P<0.01)。结论:骨碎补总黄酮具有增加动物骨密度作用,提高血钙含量,促进骨的形成,对化学刺激引起的疼痛和对热传导刺激引起的疼痛反应有显著抑制作用。

Abstract:

Objective To investigate the effects of assemble flavones of Fortune's drynaria rhizoma on animal bone mineral density,phasma calcium and phosphorus, and on analgesia action.Methods Wistar rat and Kunming mouse were used as experimental animals. Osteoporosis was induced by orally given retinoic acid(70 mg·kg^-1·d^-1) to the rats for 14 dasy. For evaluation of analgesia action,body wresting was induced by vinegar id injection in the mice, and hot plate method(55℃) of mouse was employed. Results Bone mineral density and plasma calcium were significantly increased for the dose groups of Fortune's drynaria zhizoma compared with the control(P<0.05).Analgesia action evaluated by vinegar induced and heat induced pain was also significant compared with the control(P<0.05).Conclusion The assemble flavones of Fortune's drynaria rhizoma have the functions of elevating blood calcium, increasing bone mineral density and analgesia action.