桂枝汤对高、低体温大鼠下丘脑组织转录因子CREB的影响

Effect of Guizhi Tang on CREB in Hypothalamus in Both Fever and Hypothermia Rats

摘要:

目的:探讨体温变化时下丘脑组织中磷酸化cAMP反应元件结合蛋白(pCREB)的活性及桂枝汤调节体温作用与此关系。方法:选用前列腺素受体3(EP₃)发热大鼠模型及氨基比林低体温大鼠模型,采用比色法测定给予桂枝汤后大鼠下丘脑组织中pCREB活性。结果:两种模型下丘脑组织中pCREB活性均有升高倾向,以发热大鼠明显;给予桂枝汤可使发热大鼠的pCREB活性降低,而低体温大鼠的pCREB活性变化不明显。结论:pCREB参与了大鼠的体温变化过程;桂枝汤的解热作用可能与降低pCREB活性有关;pCREB基本上不参与低体温机制的调节或仅有较弱调节作用,桂枝汤的升温作用与其关系不明显。

Abstract:

Objective:To investigate the changes of the activity of CREB in both fever and hypothermia Rats and the mechanism of thermoregulatory action of Guizhi Tang.Methods:The activity of CREB in hypothalamus in both fever and hypothermia rat pretreated with Guizhi Tang were measured via spectrophotometric method.Results:The pCREB activity in both model rats,especially in fever rats,enhanced,and treatment with Guizhi Tang counteracted the rise of pCREB in fever rats but not in hypothermia model.Conclusion:CREB may participate in the thermoregulation in rats and reduction in CREB may contributed to antipyretic action of Guizhi Tang,while the hypothermia production may be independent of CREB.