不同产地茯苓药材高效液相色谱指纹图谱的实验研究

Study on HPLC Fingerprint of Poria Grew in the Different Places

摘要:

目的:建立茯苓药材的HPLC指纹图谱。方法:以甲醇超声法制备供试液,采用HPLC法,乙腈和0.5%磷酸水为流动相梯度洗脱,紫外检测波长:242nm,柱温:30℃;流速:1.0mL/min。结果:分析了10批不同产地的茯苓药材,采用计算机辅助相似性评价系统进行了评价,建立了共有模式,并以10批茯苓指纹向量与共有模式向量间的相关系数和相合系数为测度评价指纹的相似性。结论:所建立的指纹图谱具有稳定、重现的特点可用于茯苓药材的质量控制。

Abstract:

Objective:To study the HPLC fingerprint of Poria and establish a effective method for controlling the quality.Methods:The samples were extracted with methanol and separated on a column with the mobile phase of acetonitrile-0.5%H₃PO₄ in gradient mode,and the flow rate was 1.0mL/min,The detection wavelength was 242nm and the temperature of column was 30℃.Results:The batches of Poria grew in the different places were measured.The common model was established.The correlation coefficient and cos θ between every batch vector and the total standard model vector were used to define the similarity.Conclusion:The method of fingerprint by HPLC is established and the method is stable and reproducible.It was used for quality evaluation,of the crud drug of Poria.