复方茵黄解毒汤有效部位体外抗乙肝病毒活性测定及薄层扫描指纹图谱的构建

Determination of Anti-HBV Activity in Vitro and Establishment of Thin-layer Chromatography Scan(TLCS) Fingerprinting for the Active Extract of Compound Decoction of Yinchenhao and Huanglianjiedu(CDYH)

摘要:

目的:初步构建复方茵黄解毒汤乙酸乙酯有效部位体外抗乙肝病毒活性指导下的薄层扫描指纹图谱,为复方茵黄解毒汤建立更高的质量标准打下基础。方法:以HepG₂-2.2.15细胞系为模型,采用ELISA法测定复方茵黄解毒汤的乙酸乙酯有效部位活性,再通过薄层色谱法对乙酸乙酯有效部位进行展开,运用岛津CS9000薄层扫描仪进行单波长扫描。结果:该有效部位在体外培养中对病毒抗原的分泌有较强的抑制作用。同时其薄层色谱峰也得到较好分离,获得了较理想的薄层色谱指纹图谱,并确定其中小檗碱、咖啡酸、阿魏酸和大黄酸峰的归属。结论:在活性指导下的薄层扫描指纹图谱将谱与效联系起来,可更好地体现和控制复方中药制剂的质量。

Abstract:

Objective:To establish a TLCS fingerprinting for ethyl acetate extract of CDYH with anti-HBV activity in vitro,Which will be helpful for the quality standard of CDYH.Methods:Using HepG_2-2.2.15 cells strain,the anti-HBV activity of ethyl acetate extract of CDYH was detected by ELISA,meanwhile ethyl acetate extract of CDYH was chromatographed by TLC and acaned by CS9000 TLCS.Results:The ethyl acetate extract of CDYH has a significant inhibitory effect on antigen of HBV in vitro,and A good separating TLCS fingerprinting was established and four peaks were identified as berberine,caffeic scid,ferulic acid and rhein respectively.Conclusion:The fingerprinting was connected with the effect of anti-HBV activity,and the quality of this preparation was controlled better.