不同配比黄芪汤干预大鼠胆汁淤积性肝硬化作用观察

Effect of Different Ratio of Huangqi Decoction on Cholestatic Cirrhosis in Rats

摘要:

目的:观察不同配比的黄芪汤(3∶16、∶19、∶1)干预大鼠胆汁淤积性肝硬化的药理作用。方法:结扎大鼠胆总管制备大鼠胆汁淤积性肝硬化模型;结扎1周后,将模型大鼠随机分为模型组和药物干预组,药物干预分别给予不同配比的黄芪汤经口灌胃,模型组给予等量生理盐水;用药4周后杀鼠取材。观测内容包括大鼠一般状况、组织病理、肝功能(血清ALT、AST、ALP、GGT活性及TBil、TP、Alb的含量)、肝组织羟脯氨酸(HyP)含量。结果:造模5周时大鼠胆汁淤积性肝硬化完全形成,与假手术组大鼠比较,模型组大鼠血清GGT、ALP活性及血清TBil含量、肝组织Hyp含量显著升高(P<0.05～0.01 vs sham),血清TP、Alb含量显著下降(P<0.05～0.01 vs sham)。与同期模型对照组比较,黄芪汤(6∶1、3∶1)干预治疗组肝纤维化程度显著减轻,血清ALP、GGT活性、TBil含量及肝组织Hyp含量显著降低(P<0.05～0.01 vs 5W model)。结论:黄芪汤(6∶1、3∶1)有不同程度的干预大鼠胆汁淤积性肝硬化形成的作用。

Abstract:

Objective:To investigate the effect of different ratio of Huangqi Decoction(3∶1,6∶1,9∶1) on cholestatic cirrhosis in rats.Method:Rat cholestatic cirrhosis was duplicated by ligation of bile duct.After 1 week rats were divided into model control group and different ration of Huangqi Decoction groups.The oral administration of Huangqi Decoction lasted for 4 weeks,and then rats were sacrificed for determination of liver function,liver tissue Hyp conten and histological changes.Results:cholestatic cirrhosis was successfully duplicated after 5 weeks modeling.Compared with that of normal group,serum GGT and ALP activity,serum TBiL content and liver tissue Hyp content in model group increased significantlly(P<0.05～0.01 vs sham),while serum TP and Alb content decreased significantly.Compared with that of model group,degree of cirrhosis in groups of ratio 3∶1 and 6∶1 of Huangqi Decoction alleviated significantly,serum ALP and GGT activity,serum TBiL content and liver tissue Hyp content decreased significantly(P<0.05～0.01 vs 5W model).Conclusion:The ratio of 3∶1 and 6∶1 of Huangqi Decoction inhibits formation of cholestatic cirrhosis effectively.