HPLC-ELSD法测定百草精口服液中黄芪甲苷的含量

Determination of Astragnaloside IV in Baicaojing Oral Solution by HPLC-ELSD

摘要:

目的:建立中药复方百草精口服液中黄芪甲苷的含量测定方法。方法:采用Diamonsil C₁₈色谱柱(250mm×4.6mm,5μm),乙腈-水(33∶67)为流动相,流速1.0mL·min^-1,柱温35℃,漂移管温度105℃,空气流速2.7L·min^-1。结果:黄芪甲苷线性方程为Y=1.4810X+4.6135,在(1.20～6.00)μg范围内具良好的线性关系(r=0.99997),平均回收率为99.6%,RSD为2.6%。结论:该法准确、可靠、重复性好,可有效控制百草精口服液的质量。

Abstract:

Objective:To develop an HPLC method for determin ation of astragaloside IV in Baicaojing Oral Solution.Methods: The separation was performed on a Diamonsil C₁₈(250 mm×4.6 mm,5 μm)column with acetonitrile-water(33∶67) as the mobile phase.The flow rate was 1.0 mL·min^-1.Temperature of drift tube was 105 ℃.The gas flow rate was at 2.7 L·min^-1.Results: Within the range of(1.20～6.00)μg,the content of astragaloside IV showed a good linear relationship(Y=1.481 0X+4.613 5,r=0.999 97).The average recovery of astragaloside IV was 99.6%,RSD 2.6%.Conclusion: This method is sensitive,accurate,and simple with little interference for the determination of astragaloside IV content.The method can be used for controlling the quality of Baicaijing Oral Solution.