糖肾平胶囊对糖尿病肾病大鼠肾脏保护作用及其对肾组织单核细胞趋化因子-1蛋白及mRNA表达的影响

Effects of Tangshenping Capsule on the Renal Protection and Expressions of MCP-1 and mRNA in Streptozotocin-induced Diabetic Nephropathy Rats

摘要:

目的:探讨糖肾平胶囊对糖尿病肾病大鼠肾脏保护作用及其对肾组织单核细胞趋化因子(MCP-1)蛋白及mRNA表达的影响。方法:雄性SD大鼠,50只,按体重均匀分出正常组10只,其余40只,腹腔注射链脲佐菌素造模。将造模成功的大鼠按血糖水平分为模型组、厄贝沙坦组、糖肾平胶囊组,用药12周。每周称量体重;4,8,12周后分笼收集24h尿液检测24h尿蛋白定量,处死大鼠行血液生化指标BUN、Scr、TG检测,肾组织行HE、Mallory染色,观察病理形态学改变,采用免疫组化、原位杂交分析肾组织MCP-1蛋白及mRNA的表达。结果:模型组大鼠摄食减少、精神不振、活动迟缓、尿量减少、体重减轻以及肾组织病理改变,而厄贝沙坦组和糖肾平胶囊组均有不同程度的改善,且糖肾平胶囊组优于厄贝沙坦组;正常组肾小管上皮细胞、肾间质细胞胞浆有少量MCP-1蛋白及mRNA表达;模型组则呈强阳性表达。糖肾平胶囊组MCP-1蛋白及mRNA的表达较模型组明显减少(P<0.05)。结论:糖肾平胶囊可能通过对肾组织MCP-1蛋白及mRNA表达下调而发挥治疗和预防糖尿病肾病的作用。

Abstract:

Objective:To observe the effects of Tangshenping Capsule on the expressions of MCP-1 and mRNA in streptozotocin induced diabetic nephropathy rats.Methods: 50 SD rats were randomly divided into normal control group(n=10),model group(n=40).Then the saccessfully modeled rats were re-divided into model group,irbesartan group and Tangshenping Capsule group.After administration of 12 weeks,the rats were sacrificed,Pathological changes in renal tissue were observed by HE and Mallory Staining.Using immunohistochemistry and hybridization analysis,renal tissue MCP-1 protein and mRNA expression were determined.Results: The model group showed reduction in appetite,activities,urine volume,body weight and pathological damages in kidney.Irbesartan and Tangshenping Capsule improved the above mentioned changes.Tangshenping Capsule group was superior to irbesartan group.There are some differences;in normal group and the treatment group renal tubular epithelial cells and interstitial cells showed mild MCP-1 and mRNA expression,in the model group there was a strong expression.Tangshenping Capsule significantly reduced the MCP-1 and mRNA expression(P<0.05).Conclusion: Tangshenping Capsule plays a role in treatment and prevention of DN via regulating MCP-1 and mRNA balance.