清脑宣窍滴丸对急性脑缺血损伤大鼠的保护作用及机理初探

Protective Effect of Qingnao Xuanqiao Dropping Pill on Experimental Ischemic Injury

摘要:

目的:研究清脑宣窍滴丸对急性脑缺血损伤的保护作用及机理。方法:采用线栓法所致大鼠大脑中动脉缺血再灌注(MCAO)模型,测定大鼠脑梗塞范围;测定脑匀浆中乳酸(LD)、丙二醛(MDA)、谷胱甘肽(GSH)含量及乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)活力;应用免疫组化染色的方法,观察清脑宣窍滴丸对黏附分子-1(ICAM-1)表达的影响。结果:清脑宣窍滴丸180mg·kg^-1、90mg·kg^-1可明显减小MCAO大鼠脑梗塞范围;180mg·kg^-1可明显降低MCAO大鼠患侧脑匀浆中LD含量,180mg·kg^-1、90mg·kg^-1、45mg·kg^-1可明显降低手术侧大脑组织中升高的LDH活力、提高SOD活力、降低MDA含量,180mg·kg^-1、90mg·kg^-1可明显提高手术侧大脑组织中GSH含量;180mg·kg^-1可显著降低ICAM-1阳性表达的积分光密度(IOD)。结论:清脑宣窍滴丸对脑缺血有保护作用,可能机理是通过减轻酸中毒、抑制自由基损伤、恢复自由基代谢平衡,抑制黏附分子表达来减轻缺血再灌注后炎症反应。

Abstract:

Objective:To study the role and mechanism of Qingnao Xuanqiao Dropping Pill(QNXQDP) on acute cerebral ischemia.Methods: Adopting the middle cerebral artery occlusion(MCAO)model induced by inserting a monofilament suture,the infarct size,the content of LD,MDA and the activity of GSH and LDH,SOD in brain homogenate were observed.We determined the expression of ICAM-1 in the brain influenced by QNXQDP by immunohistochemical staining method.Results:QNXQDP 180 mg·kg^-1,90 mg·kg^-1 can significantly reduce the scope of the rat MCAO cerebral infarction;180 mg·kg^-1 could significantly reduce the content of LD in the rat brain homogenate of the troubled side,180 mg·kg^-1,90 mg·kg^-1,45 mg·kg^-1 can significantly reduce the increased LDH activity,enhance the activity of SOD and reduce the content of MDA in the side of the brain surgery,180 mg·kg^-1,90 mg·kg^-1 can significantly improve the content of GSH in the side of the brain operation;180 mg·kg^-1 can significantly reduce the integral optical density of ICAM1 in positive expression.Conclusion:QNXQDP has protective effect on cerebral ischemia.The possible mechanism may be by reducing acid poisoning,inhibiting free radical damage and restoring the balance between free radical metabolism;inhibiting the expression of adhesion molecules,reducing the inflammatory response induced by ischemic reperfusion.