凉血活血方对脓毒症大鼠白细胞系列黏附分子表达的影响

Effects of Liangxuehuoxue Formula on the Expression of Multiple Adhesion Molecules in Septic Rat Model

摘要:

目的:研究凉血活血方对脓毒症大鼠白细胞黏附分子表达的影响,探讨其药理作用机制。方法:Wistar大鼠90只,雌雄各半,随机分为假手术组(10只)、模型组(20只)、凉血活血方治疗组(分为大,中,小剂量组,每组20只)。采用盲肠结扎穿孔法制作脓毒症模型,造模同时分别进行灌胃治疗,给予大,中,小剂量的活血凉血方浸膏,观察72h动物死亡率;在造模72h后腹主动脉取血抗凝,溶血素处理后,分别加入不同荧光素标记的CD11b、CD31、CD54、CD62L抗体,经流式细胞仪检测白细胞表面4种黏附分子的表达情况;行气管插管进行肺泡灌洗,收集分离白细胞,流式细胞术检测4种黏附分子的表达情况;留取肝、肺及末段回肠组织,检测髓过氧化物酶(MPO)活性。结果:模型组大鼠血中及肺泡灌洗液中白细胞黏附分子CD11b、CD31、CD54、CD62L表达较假手术组显著增高;大,中剂量凉血活血方治疗可显著降低模型大鼠血中及肺泡灌洗液中白细胞CD11b、CD31、CD54、CD62L表达,显著降低肝、肺及末段回肠组织MPO活性;凉血活血方大剂量组动物死亡率显著降低。结论:脓毒症状态下白细胞黏附因子的异常高表达及其与血管内皮的黏附作用可介导多种脏器功能障碍,活血凉血中药可通过抑制血管内皮系列黏附分子高表达而发挥治疗作用。

Abstract:

Objective:To study the effects of Liangxuehuoxue Formula on the the expression of multiple adhesion molecules in treating a cecal ligation and puncture(CLP) rat septic model.Methods:Using a sepsis model induced by CLP,the septic rats were treated with 3 different doses of liquid extract of Liangxuehuoxue Formula for 72 hours.The expressions of CD11b,CD31,CD54 and CD62L in leucocytes of circulating blood and bronchoalveolar lavage fluid(BALF) were determined by flowcytometry.Myeloperoxidase(MPO) activity of lung,liver and intestinal tissues were examined.Results:The 72 h mortality of model group was 70% and the expressions of CD11b,CD31,CD54 and CD62L in leucocytes of circulating blood and BALF and MPO of lung,liver and intestinal tissues in model group were all increased significantly compared with sham operation group.The high dose of Liangxuehuoxue Formula decreased the 72 h mortality to 20%,significantly lower than that of model group.The high and middle doses of Liangxuehuoxue Formula reduced the CD11b,CD31,CD54 and CD62L expressions in leucocytes of circulating blood and BALF and MPO of lung,liver and intestinal tissues significantly.Conclusions:The high expressions of adhesion molecules and their adhesive effects on the endothelium play an important role in the pathological process of multiple organ dysfumctions in sepsis and the Liangxuehuoxue Formula has therapeutic effects on septic model by reducing the high expressions of multiple adhesion molecules.