20种中药水溶性糖GC-MS指纹图谱与寒热药性的Fisher判别分析

The Fisher Analysis Could Distinguish Relationship between Cold and Heat Properties and GC-MS Fingerprints for Water-Soluble Sugar of 20 Chinese Herbs

摘要:

目的：探讨中药寒热药性与水溶性糖的相关性，进一步阐明中药四性与物质基础的关系。方法：选取10种寒性中药和10种热性中药，提取水溶性糖，分别加入盐酸羟胺、吡啶和乙酸酐进行乙酰化反应，测定水溶性糖的GC-MS指纹图谱，通过质谱确定各味中药的水溶性糖类成分，利用Fisher方法建立判别函数，分析实验结果。结果：建立寒热药性的Fisher判别函数，实现了对20种中药寒热药性的准确识别，组内回代一致率为100%。结论：Fisher可以作为判别中药寒热药性与水溶性糖关系的统计学工具，水溶性糖是中药寒热药性的物质基础之一。

Abstract:

Objecive To discuss the relationship between water-soluble sugar and cold-heat properties and furtherly reveal correlations of four properties and material basis.Methods Ten cold herbs and heat herbs were selected for the determination,and dealed with the water-soluble sugar with hydroxylamine hydrochloride,pyridine and acetic anhydride.Measuring Gas chomatography-mass(GC-MS) fingerprints of water-soluble sugar,then determining the water-soluble components of 20 herbs by mass spectrometry and analyzing the results by Fisher method.Results A discriminant function had been established by Fisher.The Fisher discriminant analysis could distinguish the cold and heat properties about 20 herbs that the group back substitution is 100%.From the results,it confirmes that there is difference between the cold and heat properties in the GC-MS fingerprints by Fisher method,which shows that there are different water-soluble sugar in the cold and the heat herbs.Conclusion The Fisher analysis could differentiate the cold and heat properties of herbs,and it is an effective statistical tool to study the relationship between the cold and heat properties and water-soluble sugar.It concludes that the water-soluble sugar is one of the material basis of four properties of herbs.