灯盏花素对缺血再灌注大鼠心肌超微结构及细胞凋亡的保护作用

Protective Effect of Breviscapine Against Myocardial Ischemia Reperfusion on Myocardial Cell Ultrastructure and Apoptosis in Rats

摘要:

摘要 目的：探讨灯盏花素(breviscapine)对缺血再灌注大鼠心肌的保护作用及其机制。方法：40只SD大鼠随机分为假手术组，缺血再灌注组（IR组）， 灯盏花素组。灯盏花素组大鼠于术前1周分别腹腔注射不同剂量的灯盏花素（25、50 mg.kg-1.d-1），另2组给等量生理盐水。制备心肌缺血再灌注模型。采用Annexinv-PI双染，流式细胞仪检测心肌细胞的凋亡情况；电镜观察各组大鼠心肌超微结构的变化。结果：灯盏花素可明显降低缺血再灌注大鼠心肌细胞的凋亡率（P＜0.05，P＜0.01），且较IR组心肌细胞超微结构损伤程度减轻。结论：灯盏花素对缺血再灌注心肌细胞具有保护作用，其机制可能是通过抑制心肌细胞凋亡来实现的。

Abstract:

ABSTRACT: Objective: To explore protective effect and mechanism of breviscapine against myocardial ischemia reperfusion injury in rats. Methods: 40 SD rats were divided into sham operation group , ischemia reperfusion group and breviscapine group randomly.Rats in breviscapine group were injected with breviscapine 25、50mg.kg1.d-1 respectively prior to ischemia reperfusion .other two groups were given NS instead of breviscapine equally at a week before the operation. Myocardial ischemia reperfusion injury model was established. Apoptosis of myocytes was detected after operation in all groups by flow cytometry with AnnexinⅤ-EGFPand propidium iodiode ( PI) staining. The ultrastructural alterations were observed under the transmission electron microscopy. Results : breviscapine could decrease the occurrence rate of myocardial apoptosis during ischemia reperfusion injury obviously（P＜0.05，P＜0.01）;The degree of injuring on ultrastructures of myocardial cell in breviscapine group is rather alleviated than in ischemia reperfusion group.Conclusion : breviscapine may protect myocardial cells during ischemia reperfusion injury and the protective mechanism may be obtained by inhibiting myocardial apoptosis.