RP-HPLC法测定维感泡腾片中腺苷的含量

Determination of Adenosine in Weigan Effervescent Tablets by RP-HPLC

摘要:

目的:采用RP-HPLC法建立维感泡腾片中腺苷的含量测定方法。方法:以甲醇-水(8∶92)为流动相,ShimadzuC₁₈(250 mm×4.6 mm,S-5 μm)色谱柱,检测波长为260 nm,柱温为35 ℃,流速为1.0 mL·min^-1。 结果:腺苷在4.3~33.8 μg范围内呈良好的线性关系(r=0.999 7),腺苷的平均回收率为101.0%(n=9,RSD=1.4%)。结论:该方法快速简便易行,重复性良好,结果准确,可作为维感泡腾片中腺苷质量评价的方法。

Abstract:

Objective:To develop a RP-HPLC method for the quantification of adenosine in Weigan Effervescent Tablets. Methods: The analysis was carried out on a Shimadzu C₁₈ column(250 mm×4.6 mm,S-5 μm)and the mobile phase was a mitture of methanol-water(8∶92)with a flow rate of 1.0 mL· min^-1.The detection wavelength was 260 nm, and the column temperature was 35 ℃. Results: The linear response ranges of adenosine was 4.3~33.8 μg(r=0.999 7), and the average recovery was 101.0%(n=9,RSD=1.4%). Conclusion: The method is quick, simple and reproducible for the determination of adenosine in Weigan Effervescent Tablets.