基于HPLC-DAD-MS的当归补血汤化学成分分析

Chemical Composition Analysis of Dangguibuxue Decoction by HPLC-DAD-MS

摘要:

目的 :分析中药复方当归补血汤的化学成分。 方法 :采用HPLC-DAD-MS方法分析。色谱柱为Thermo Hypersil-HypurityC₁₈(150 mm×2.1 mm, 5 μm)分析柱,柱温40 ℃。流动相为H₂O-CH₃OH(梯度洗脱),流速为0.2mL·min^-1。二极管阵列扫描范围:200~370 nm。质谱采用大气压化学电离(APCI+)离子源,扫描范围:100~650(m/z)。 结果 :通过与已有的文献报道的质谱、紫外光谱和保留行为比较可以初步定性10个化合物。 结论 :因为能够同时提供分子量、紫外光谱和保留行为,所以LC-DAD-MS是一个分析中药复方化学成分的有力工具。

Abstract:

Objective: To study chemical components of Dangguibuxue decoction. Methods :The compounds were separated on a Hypersil-Hypurity C₁₈(150 mm×2.1 mm, 5 μm) analytical column. The column temperature was set at 40 ℃. Mobile phase consisted of methanol and water using a gradient elution. The flow rate was set at 0.2 mL·min^-1. UV spectra were obtained by scanning from 200 nm to 370 nm. The APCI-MS spectra were obtained in the positive ion mode scanning from m/z 100 to 650. Results :The peaks were identified by comparing their UV spectra, their molecular weights and their retention behaviors with data reported in the literature. The ten components seem to be tentatively identified. Conclusions: Because of providing UV and molecular mass as well as retention time, LC-DAD-MS is a powerful tool for the chemical analysis of Traditional Chinese Medicines.