HPLC法测定舒利通颗粒中巴马丁和小檗碱的含量

Dertermination of Palmatine and Berberine in Shulitong Granule by HPLC

摘要:

目的: 建立以高效液相色谱法测定舒利通颗粒中巴马丁和小檗碱含量的方法。 方法: 色谱柱:Kromasil-C₁₈(250 mm×4.6 mm, 5 μm);流动相:乙腈-0.1 mol·L^-1磷酸二氢钾溶液(28 ∶72);流速:1 mL·min^-1;检测波长:345 nm;柱温:室温。 结果: 盐酸巴马丁在0.016~0.080 μg范围内(r=0.999 8),盐酸小檗碱在0.027~0.135 μg 范围内(r=0.999 9)呈良好的线性关系。盐酸巴马丁平均回收率为99.74%, RSD=2.67%(n=5);盐酸小檗碱平均回收率为103.53%,RSD=1.48%(n=5)。 结论: 此方法简捷、准确性、重复性好、专属性强,可供用于舒利通颗粒的质量标准研究。

Abstract:

Objective: To develop a HPLC method for the determination of palmatine and berberine in Shulitong Granule. Methods: A Kromasil-C₁₈(250 mm×4.6 mm, 5 μm)column was used for the HPLC determination.The mobile phase consisted of acetonitrile-0.1 mol/L potassium dihydrogen phosphate (28 ∶72),and the flow rate was 1 mL/min.The detection wavelength was 345 nm. Results: A good linear relationship was constructed in the range of 0.016~0.080 μg (r=0.999 8), for Palmatine hydrochloride and in the range of 0.027~0.135 μg (r=0.999 9) for berberine hydrochloride. The average recovery of palmatine hydrochloride was 99.74% (RSD=2.67%, n=5),and that of berberine hydrochloride was 103.53%,RSD=1.48%(n=5). Conclusion: The developed method is simple, reproducible, specific and suitable for the quality control of Shulitong Granule.