还脑益聪方对老龄大鼠学习记忆及脑组织β类淀粉样蛋白和P物质水平的影响

Effects of Huannao Yicong Prescription on Learning and Memory, Level of Brain β-amyloid Protein and Substance P in Aging Rats

摘要:

目的: 观察还脑益聪方对老龄大鼠学习记忆功能及脑组织β类淀粉样蛋白(β-AP)和P物质(S.P)水平的影响。 方法: 以Morris水迷宫试验检测老龄大鼠的空间学习记忆能力,将学习记忆减退的40只大鼠用随机表分成4组,每组10只。即模型(记忆减退老龄大鼠)对照组、还脑益聪方大剂量(13.68 g生药/kg)组、小剂量(6.84 g生药/kg)组和阳性药物对照组(盐酸多奈哌齐片,0.45 mg/kg)组,另取学习记忆能力正常老龄大鼠10只为正常对照组,给与等量水,其余灌胃给药8周。观察测定老龄大鼠的空间学习记忆能力和脑组织匀浆测定β-AP和P物质含量,并检测大鼠血清超氧化物岐化酶(SOD)和丙二醛(MDA)的含量,以HE染色观察大鼠脑组织形态学的病理变化。 结果: 模型组老龄大鼠在水迷宫中的游泳时间、路径长度明显延长。给药8周后,还脑益聪方大、小剂量组大鼠游泳持续时间、路径长度较模型组显著缩短,寻找平台的方式有所改进。和正常老龄大鼠组比较,模型组大鼠MDA含量显著升高,SOD活力显著降低。与模型组比较,给药组大鼠脑组织的β-AP含量下降和P物质水平增加,血清SOD水平显著升高(P<0.01),脑组织形态结构得到改善。 结论: 还脑益聪方可降低β-AP在老龄大鼠脑组织的沉积,保护脑组织海马形态的完整性,从而改善老龄大鼠空间学习记忆能力。

Abstract:

Objective: To observe the effects of Huannao Yicong Prescription(HYP) on learning and memory impairment,level of brain β-amyloid Protein (β-AP)and substance P(S.P)in aging rats. Methods: Morris water maze test was used to measure the spatial learning and memory of rats. 40 rats with learning and memory impairment were divided randomly into four groups (10 in each group):the model group (learning and memory impairment aged rats group),the high-dose HYP group (13.68 g crude drug/kg),the low-dose HYP group (6.84 g crude drug/kg),the positive control group (Hydrochloricdonepezil Tablets 0.45 mg/kg),besides,a normal aged rats group with 10 rats was set up,they were given tales doses of distilled water,all other groups were orally administered with drugs for 8 weeks. Then the spatial learning and memory of rats were measured again by Morris water maze. Rats were sacrificed after anesthesia and the brain homogenate was made for measuring the content of β-AP and S.P. The activity of superoxide dismutase (SOD) and the content of maleic dialdehyde (MDA) in serum was measured,the histomorphological changes in the brain were observed after hematoxylin eosin (HE) staining. Results: The latency and path length in water maze test in the model group were significantly prolonged. After 8 weeks of administration,the latency and path length in both of the HYP groups were significantly shortened than the model group,and the strategy in finding the platform was improved. Compared with the normal aged rats group,the content of MDA was significantly increased while the activity of SOD was significantly decreased in the model group. After 8 weeks of administration,compared with the model group,the level of β-AP was decreased while the S.P was increased,the activity of SOD was significantly increased(P<0.01)and the histomorphology in the brain was improved in the HYP groups. Conclusion: HYP can reduce the deposition of β-AP in the brain tissue,protect the integrity of histomorphology in the hippocampus,and thus improve the spatial learning and memory for aged rats.