寒热性中药的成分对薄荷醇受体离子通道蛋白功能的影响

ESR study and Protection of Water Extract of Carthamus tinctorius L. on Injury of Cardiomyocytes induced by Hydrogen Peroxide

摘要:

目的: 探讨寒热中药成分对瞬变感受器离子通道蛋白(TRP)家族中薄荷醇受体离子通道蛋白(TRPM8)功能的影响。 方法: 以薄荷醇为通道激动剂,采用共聚焦显微成像法在体外观察原代培养背根神经节(DRG)神经元胞内 _i的变化。 结果: 寒性中药的成分黄芩甙和大黄素均可显著上调TRPM8通道蛋白的功能;热性中药的成分桂皮醛可显著下调TRPM8通道蛋白的功能,热性成分吴茱萸碱对TRPM8通道蛋白功能虽未见显著影响,但有下调趋势。 结论: 寒热性中药成分对TRPM8功能的调节可能与中药的寒热药性相关,这可能是寒热性中药临床上发挥寒热调节作用的分子机制之一。

Abstract:

Objective: To investigate the antioxidant effects of water extract of Carthamus tinctorius L. on injury of cardiomyocytes induced by hydrogen peroxide (H₂O₂) and to detect reactive oxygen species (ROS) for exploring the mechanisms. Methods: In cultured neonatal rat cardiomyocytes, the antioxidant effect of water extract of Carthamus tinctorius L. was investigated after H₂O₂ (1 mmol·L^-1) induced damage. The spontaneous beating, the survival rate and release of LDH, MDA, SOD, NO, NOS, GSH-Px and XOD enzyme activity in supernatant were investigated, and cell suspension was collected for detecting reactive oxygen species (ROS) by electron spin resonance (ESR). Results: Preconditioning by water extract of Carthamus tinctorius L. increased the spontaneous beating and the survival rate, and decreased the release of LDH, MDA and SOD with the rise of SOD, NO, NOS and GSH-Px enzyme activity, while in the cell suspension ROS signal decreased significantly. Conclusion: Water extract of Carthamus tinctorius L. has antioxidative effect. The mechanisms are likely related with scavenging of free radicals, enhancing its clearance, enhancing endogenous antioxidant activity.