不同类型醋对小鼠的急性毒性及炮制延胡索镇痛作用比较

Protective Effects of Water Extract of Rhizoma Coptidis and Fructus Evodiae on Ethanol-induced Rat Gastric Lesions

摘要:

目的: 探讨不同类型醋炮制延胡索镇痛作用;对不同类型醋进行安全性比较,为筛选炮制辅料用醋提供依据。 方法: 采用前实验筛选出的6种醋炮制延胡索,比较其镇痛作用;采用不同类型的醋比较对小鼠单次ig的毒性。 结果: 各醋炮制品组与对照组比较均有明显的镇痛作用,组间比较无明显差异;小鼠ig食用米醋未见毒副反应,工业醋酸及用食用醋酸配制的白醋,各组小鼠ig醋72 h内均出现不同程度异常反应及死亡。 结论: 筛选的6个品牌醋可作为延胡索炮制辅料用醋,6种醋炮制延胡索镇痛作用无显著差异;工业醋酸及用食用醋酸配制的白醋较发酵米醋安全性差。

Abstract:

Objective: To investigate the protective effects of water extract of Coptis chinensis and Evodia rutaecarpa on ethanol-induced acute gastric lesions in rats. Methods: In this study, animals were intraperitoneally pretreated with vehicle (normal saline) or Coptis chinensis and Evodia rutaecarpa water extract at 1050 (6 ∶1), 1800 (6 ∶6), 1050 (1 ∶6) mg·kg^-1. Three hours later, gastric lesions were induced by topical application of 50% ethanol for 10 min. The length index of gastric lesions were determined by morphological analysis in a gastric intubatton model. The rat gastric transmucosal potential difference (PD) was recorded continuously by ex-vivo chamber technique. The pH value and H⁺ content of gastric perfusate were mesured by pH meter and pH-stat technique, respectively. Results: Compared with control, Coptis chinensis and Evodia rutaecarpa water extract (1 ∶6) accelerated PD recovery and reduced gastric morphologic lesions. Coptis chinensis and Evodia rutaecarpa water extract (1 ∶6) elevated H⁺ content and reduced pH value. Coptis chinensis and Evodia rutaecarpa water extract (6 ∶6) reduced gastric morphologic lesions. Conclusions: Coptis chinensis and E. rutaecarpa water extract (1 ∶6) showed effectively protective actions on ethanol-induced acute gastric lesions in rats, and the gastroprotective mechanisms maybe due to the strengthening action on gastric mucosal lining and the inhibition of acid backdiffusion. This investigation also indicated that E. rutaecarpa maybe have a critical role in gastroprotective effect.