当归-川芎药对不同配比组方对家兔血小板聚集和凝血功能的影响

Effect of Radix Angelica and Rhizoma Ligusticum with Different Proportions and Preparation Methods on Platelet Aggregation and Prothrombin

摘要:

目的: 观察当归-川芎不同配比(1 ∶0,2 ∶1,1.5 ∶1,1 ∶1,1 ∶1.5,1 ∶2,0 ∶1)分别经3种制备方法(水提、醇提、先水提后醇提)提取后所得药液对血小板聚集和凝血酶原时间(PT)、凝血酶时间(TT)的影响,探讨当归-川芎(归芎)药对因配比组方的不同而产生功效的差异性。 方法: 家兔颈动脉取血,比浊法测定ADP诱导的血小板聚集率和PT,TT,并比较归芎药对对以上各指标的影响。 结果: 归芎药对各组均显示一定的抗血小板聚集和抗凝血作用(P<0.01或P<0.05)。归芎不同配比药对中1.5 ∶1对血小板聚集的作用弱于其他配比样品,对PT,TT的影响则是随着当归在药对中比例的增大而增强;3种不同的提取方法中,水提液对血小板聚集作用较弱,醇提液对PT影响较强而对TT影响较弱。 结论: 当归-川芎不同配比与不同制法所产生的抗血小板聚集和抗凝血作用不同,并可能与其功效差异相关,其内在的物质基础与相关机理值得深入研究。

Abstract:

Objective: To observe the effect of Radix Angelica and Rhizoma Ligusticum with different proportions (1 ∶0,2 ∶1,1.5 ∶1,1 ∶1,1 ∶1.5,1 ∶2,0 ∶1) extracted by different methods (by water、by ethanol、firstly by alcohol then by water) on platelet aggregation and prothrombin time (PT), thrombin time (TT). Methods: Take blood from carotid artery of rabbit and measure the rate of platelet aggregation induced by ADP and PT, TT to compare their different effects. Results: Radix Angelica and Rhizoma Ligusticum with different proportions and preparation methods showed certain effects of anti-platelet aggregation and antithrombin (P<0.01 or P<0.05). The effect on PT and TT enhanced along with the ratio of Radix Angelica gradually increases; The platelet aggregation effect was the weakest While the ratio of Danggui-Chuanxiong was 1.5 ∶1. Compared with other extracts, the solution extracted by water showed a weaker effect of platelet aggregation, while solution extracted by ethanol showed a stronger effect on PT than TT. Conclusion: The different traditional efficacy of Radix Angelica and Rhizoma Ligusticum with different proportions and preparation methods may be related with their different effects of anti-platelet aggregation and antithrombin, their intrinsic material basis and relevant mechanism are worthy of further study.