加味生脉散对急性心肌缺血再灌注损伤大鼠的保护作用

Protective Effect of Jiawei Shengmaisan Against Acute Myocardial Ischemia/Reperfusion Injury in Rats

摘要:

目的: 研究加味生脉散对急性心肌缺血再灌注损伤大鼠的保护作用。 方法: 采用冠状动脉结扎法复制急性心肌缺血再灌注损伤大鼠模型,经十二指肠给予不同剂量的加味生脉散水煎液,NBT染色法检测心肌梗死面积,计算心肌梗死程度;检测血清SOD活性与MDA,NO含量。 结果: 加味生脉散能明显降低大鼠心肌梗死程度,降低血清MDA含量,升高血清SOD活性与NO含量。 结论: 加味生脉散对急性心肌缺血再灌注损伤大鼠具有一定的保护作用,该作用可能与其抗氧化作用、升高血清NO含量有关。

Abstract:

Objective: To investigate protective effect of Jiawei Shengmaisan against acute myocardial ischemia/reperfusion injury in rats. Methods: The acute myocardial ischemia/reperfusion injury in rats was induced by ligating the anterior descending coronary artery in SD rats and administered different dosage jiawei shengmaisan decotion into duodenum, myocardial infarction degree(MID) was calculated by detcting myocardial infarction area with nitroblue tetrazolium(NBT) assay,SOD activities and MDA、NO contents were detected with biochemical assay. Results: Jiawei shengmaisan can significantly decreased MID and MDA contents in serum,increased SOD activities and NO contents in serum. Conclusions: The results showed that Jiawei shengmaisan has protective effect against acute myocardial ischemia/reperfusion injury in rats,which may be relate to its antioxygen action and NO.