复方丹参注射液Ⅱ中丹酚酸B在大鼠体内的HPLC测定

Effect of Cyclovirobuxine D on the Action Potentials ofVentricular Myocytes in Guinea Pig

摘要:

目的 :探讨复方丹参注射液Ⅱ的制备及其指标成分丹酚酸B在大鼠体内的含量测定方法。 方法 :通过羟丙基-β-环糊精包合增加降香挥发油溶解性;用HPLC法测定主成分丹酚酸B在大鼠体内的含量。 结果 :成功制备复方丹参注射液Ⅱ,建立注射液中指标成分丹酚酸B在大鼠体内的HPLC测定方法。 结论 :复方丹参注射液Ⅱ的制备工艺可行,指标成分丹酚酸B在大鼠体内的含量测定方法准确可靠。

Abstract:

Objective: To investigate the effect of Cyclovirobuxine D(CVB-D) on action potentials in isolated guinea pig ventricular myocytes. Methods: The single myocytes were enzymatically obtained from the guinea pig hearts and the action potentials were examined by whole-cell patchclamp technique. Results: 4×10^-4 g/L CVB-D decreased action potential amptitude(APA)from 113.3±6.3 mV to 110.2±6.4 mV(n=6, P<0.01), prolonged action potential duration of repolarization 50%(APD₅₀)from 616.3±68.0 ms to 657.4±79.5 ms(n=6, P<0.01),prolonged action potential duration of repolarization 90%(APD₉₀)from 633.5±71.2 ms to 669.2±74.4 ms(n=6, P<0.01), Prolonged action potential duration of repolarization 50% to repolarization 90%(APD_50-90)from 15.9±6.0 ms to 20.1±5.2 ms(n=6, P<0.01),and decreased decay slope of phase 3 (V₃) from -2.6±0.8 V/s to -2.4±0.7 V/s(n=6, P<0.01).Compared with the baseline,4×10^-3 g/L CVB-D makes the APD_50-90 pass to increase trend gradually,but the variety trend of the V₃ decrease gradually, other index sign varieties are consistent. Conclusion: CVB-D decreased APA and V₃, and prolonged APD₅₀,APD₉₀,APD_50-90,which may be its mechanism of positive inotropic effect and antiarrhythmic effect.