茜草中Lucidin,Lucidin-3-O-primeveroside及大叶茜草素在小鼠血浆中代谢趋势的初步研究

Experimental Studies on the Treatment of Type Ⅱ Collagen Induced Arthritis with Da-Fang-Feng-Tang

摘要:

目的: 研究中药茜草中遗传毒性成分Lucidin及其苷Lucidin-3-O-primeveroside(EKU-4)在动物体内的代谢,对茜草的用药安全性进行初步考察。 方法: 采用反相高效液相法测定茜草70%乙醇提取物以及ig该提取物一定时间的动物血浆样品。 结果: 茜草提取物中均能够检测到Lucidin、EKU-4 及大叶茜草素;茜草70%乙醇提取物,经ig给药后,血浆样品中仅能检测到Lucidin及大叶茜草素,并且给药15 min,Lucidin的血药浓度达到最高,给药后30 min,大叶茜草素的血药浓度达到最高。 结论: 由于ig给药前后血浆中未检测到EKU-4,而Lucidin成分有增加的趋势,因此推断中药茜草中EUK-4成分可能分解为Lucidin的形式参与动物体内代谢。

Abstract:

Objective: To observe the effect of Da-Fang-Feng-Tang(DFFT) in treating of Rheumatoid Arthritis(RA). Methods : The animal model of type Ⅱ collagen induced arthritis was used. Granules of the DFFT extract were administered by gastric gavage, studying in genetics, immunology, physiology and clinical. Results : The results indicated a higher onset rate, a severer redness and swelling for arthritic limb and a longer time of clinic manifestation in contract group than treated group, and the IgG level in sera was significantly increased compared contract group to treated group(P<0.05). The gene express of IL-1, TNF-α and IFN-γ were higher in contract group. Conclusion : DFFT express obviously suppressive function on model of type Ⅱ collagen induced arthritis in NIH mouse, and support the belief that DFFT is effective in treating human RA.