清热化瘀滋阴方对狼疮患者外周血淋巴细胞凋亡及相关基因表达的影响

The Effect of Qingrehuayuziyin Prescription on Lymphocyte Apoptosis and Expression of Fas and Bcl-2 in Peripheral Blood in the Patients with Systemic Lupus Erythematosus

摘要:

目的 :探讨中药清热化瘀滋阴方对系统性红斑狼疮(SLE)患者外周血淋巴细胞凋亡及相关基因(Fas,Bcl-2)表达的影响。 方法 :临床筛选SLE患者80例,完全随机分为治疗组(清热化瘀滋阴方配合激素组)和对照组(单用激素组)各40例,服药3个月,淋巴细胞分离液分离治疗前后SLE患者外周血淋巴细胞,采用流式细胞仪检测淋巴细胞凋亡率;采用Rt-PCR法检测淋巴细胞Fas,Bcl-2基因表达情况。 结果 :清热化瘀滋阴方治疗后SLE患者外周血淋巴细胞凋亡率较治疗前明显下降(P<0.01),并明显低于对照组(P<0.01);两组治疗后Fas,Bcl-2基因的表达均明显下降,治疗组Fas基因的表达明显低于对照组(P<0.01)。 结论 :清热化瘀滋阴方可能通过降低SLE患者外周血淋巴细胞凋亡率,下调淋巴细胞Fas,Bcl-2基因的表达水平,改善免疫紊乱而发挥作用。

Abstract:

Objective: To investigate the effect of Qingrehuayuziyin prescription on lymphocyte apoptosis and expression of Fas and Bcl-2 in peripheral blood in the patients with systemic lupus erythematosus (SLE). Methods : Eighty patients with SLE were selected and divided randomly into two groups, 40 in the therapy group and 40 in control group respectively. Patients were got the treatment of Qingrehuayuziyin prescription combined hormone as therapy group and got hormone treating only as control group. After treating three months, the patients lymphocyte were collected and the rate of apoptosis were detected by flow cytometer. Meanwhile, the expression of Fas and Bcl-2 were analyzed by RT-PCR. Results: After 3 months treating, the rate of lymphocyte apoptosis was decreased significantly in therapy group compared with that in control group and with that in pre-treating(P<0.01). The expression of Fas and Bcl-2, compared with that in control group, were also decreased significantly (P<0.01). Conclusion : Qingrehuayuziyin prescription is useful for treating SLE by suppressing lymphocyte apoptosis ,decreasing the level of Fas and Bcl-2 in peripheral blood and further modifying the immune status.