吴茱萸汤大孔树脂精制工艺

Study on Refining Technology of Wuzhuyu-tang by Macroporous Resin

摘要:

目的 :研究大孔吸附树脂富集吴茱萸汤水煎液中7种有效成分的工艺条件。 方法 :以柠檬黄素-3-O-β-D-葡萄糖苷(Lcs)、人参皂苷Re(Re)、人参皂苷Rb₁(Rb₁)、吴茱萸内酯(Li)、姜辣素(6-Gi)、吴茱萸碱(Ev)、吴茱萸次碱(Ru)等7种成分为考察指标,通过静态及动态吸附考察,确定了筛选的树脂型号;采用单因素实验考察了吸附时间、上样量与树脂体积比;采用正交实验,对工艺参数进行了优化。 结果 :正交实验表明,影响吴茱萸汤大孔树脂精制工艺的主要因素为径高比、上样药液浓度和乙醇浓度,最佳工艺为径高比1 ∶4,上样药液浓度0.33 g/mL,上样流速0.1倍柱体积/min,60%乙醇洗脱。 结论 :吴茱萸汤中7种成分精制后含量保留70%以上,其中5种成分保留90%以上,用大孔树脂对吴茱萸汤水提液进行精制是合理可行的。

Abstract:

Objective: To set up a proper technology of refining of the aqueous extract of Wuzhuyu-tang. Method: 7 kind components which had been improved to be absorbable pharmacological ingredients were chosen as index for refining. The static adsorption,dynamic adsorption and leakage curve experiments were done to choose a proper resin. By means of single-factor experiment and orthogonal experiment,the optimum refining conditions were found,such as adsorption time, proportion of sample weight and resin volume,proportion of diameter and height,concentration of decoction and eluent,flow rate,eluent volume and so on. Result: The AB-8 resin had the best separating efficiency when the content in the aqueous extract was 0.33g per 1mL equivalent to raw material,the ratio of dia-height was 1:4,and the volume of 60% ethanol as eluant 8BV with desorption-power 0.1BV/min. Conclusion: The content of 7 kind components were over 70% after refining, 5 of them were over 90%. It is concluded that the optimized extracting and refining technology is reasonable and feasible.