不同产地丹参药材红外光谱分析

Salviae Miltiorrhizae Radix et Rhizoma by Infrared Spectroscopy

摘要:

目的: 建立红外光谱技术,快速鉴别丹参药材产地及确定丹酚酸B含量高低。 方法: 采集来自4个产地的40批丹参药材的红外光谱信息,寻找可以反映产地特征的二阶导数红外光谱特征峰,以及可进行丹酚酸B快速定量的原谱特征峰。 结果: 不同产地丹参药材的二阶导数红外光谱特征吸收峰存在差异,可以反映丹参药材的不同产地;原谱1 262 cm^-1峰的吸收峰高基本不受其他成分的影响,可以反映丹酚酸B含量且与液相色谱含量结果呈正相关,实现丹酚酸B的快速半定量。 结论: 建立的红外光谱技术可以为中成药生产过程中,投料丹参药材的快速评价提供一种新的备选方法。

Abstract:

Objective: To establish a fast method to evaluate the geographic origins of salviae miltiorrhizae radix et rhizoma and the contents of salvianolic acid B based on infrared spectroscopy (IR). Method: IR spectra of forty samples collected from 4 different origins were analyzed to search for the characteristic peaks on second derivative (2D) spectra to reflect the characteristics of origin, also to search for a characteristic peak on normalized spectra for fast quantitation of salvianolic acid B. Result: Some characteristic peaks on 2D spectra were found which reflect 4 different origins. The intensity of 1 262 cm^-1 on normalized spectra was found to correlate well with the content of salvianolic acid B determined by high performance liquid chromatography, which realize the fast quantitative analysis of salvianolic acid B. Conclusion: The proposed IR method provides a new alternative of fast quality evaluation of salviae miltiorrhizae radix et rhizoma for manufacture of traditional Chinese medicine.