HPLC测定排氚片中黄芪甲苷的含量

Determination of Astragaloside Ⅳ in Paichuan Tablets by HPLC

摘要:

目的 :建立排氚片中黄芪甲苷的HPLC含量测定方法。 方法 :采用高效液相色谱法,色谱柱:Diamonsil C₁₈(4.6 mm×250 mm,5 μm),流动相为乙腈-水(32 ∶68),漂移管温度50 ℃,载气压力2.0 bar,蒸发光散射检测器,流速:1.0 mL ·min-1。 结果 :黄芪甲苷在0.41~2.45 μg范围内呈现良好的线性关系(r=0.999 9),加样回收率100.04%,RSD为0.13%。 结论 :该方法快速、简便、准确、重复性较好,结果可靠,可用于控制排氚片中黄芪甲苷的含量。

Abstract:

Objective: To establish an accurate method for the determination of astragaloside Ⅳ in Paichuan Tablets. Method: Diamonsil C₁₈(4.6 mm×250 mm,5 μm),with a mobile phase consisting of acetonitrile-H₂O(32 ∶68),the detector is Evaporative Light-scattering Detector,The drift tube temperture of 50 ℃,the flow rate was 1.0 mL ·min^-1. Result :A good linearity of astragaloside Ⅳ was obtained within the range of 0.41~2.45 μg, the average recovery was 100.04% and RSD was 0.14%. Conclusion :The method was simple and accurate, and can be used for the quality control of Paichuan Tablets.