反相高效液相色谱法测定蒲公英中齐墩果酸的含量

Determination of Oleanolic Acid in dandelion by RP-HPLC

摘要:

目的 :建立蒲公英中齐墩果酸的高效液相含量测定法。 方法 :采用高效液相色谱法,Hypersil ODS₂ C₁₈色谱柱(4.6 mm×250 mm,5 μm),柱温30 ℃,以甲醇-0.5%磷酸 (pH=3.5)(90 ∶10) 为流动相,流速1.0 mL ·min^-1检测波长210 nm。 结果 :齐墩果酸在0.872~7.848 μg范围内峰面积与进样量有良好的线性关系,回归方程为:Y=6.937 3X-1.989 4, r=0.999 9,平均回收率为98.25%,RSD为1.54%。 结论 :该法简便快速、准确,重复性好,有助于蒲公英质量控制方法的研究。

Abstract:

Objective: To establish the determination method for oleanolic acid in dandelion by HPLC. Method: HPLC was carried out on Hypersil ODS₂ C₁₈(4.6 mm×250 mm,5 μm)at 30 ℃ using methanol-0.5% H₃PO₄ (pH=3.5)(90 ∶10) as mobile phase with a flow rate of 1.0 mL ·min^-1 and detection wavelength was at 210 nm. Result :The oleanolic acid was linear within the range of 0.872~7.848 μg. The average recovery was 98.25% and RSD was 1.54%. Conclusion :This method is useful for quality control of dandelion.