薄层扫描法测定广西匙羹藤中牛弥菜醇A的含量

Determination of Conduriol A in Guangxi Gymnema sylvestre( Retz.)Schult by TLCS

摘要:

目的 :采用薄层扫描法测定广西匙羹藤 Gymnema sylvestre (Retz.)Schult中牛弥菜醇A的含量。 方法 :使用硅胶H板,展开剂为苯-乙酸乙酯-甲醇(10 ∶3 ∶5), 5%香草醛硫酸溶液显色,于105 ℃加热至斑点清晰,在200 nm~800 nm范围内进行扫描,检测波长538 nm,参比波长700 nm。 结果 :牛弥菜醇A的线性范围为1.025~6.150 μg,平均加样回收率为98.84%, RSD=2.02%(n=6),匙羹藤中牛弥菜醇A的含量为2.08%。 结论 :该法简便、灵敏、准确性高,重复性好,可为匙羹藤药材的定性鉴别和含量测定提供科学依据。

Abstract:

Objective: To determine the content of conduriol A in Guangxi Gymnema sylvestre (Retz.)Schult by TLCS. Method :Silica H thin layer plate was used; the developer reagent was benzene:ethyl acetate:methanol(10 ∶3 ∶5), the color reagent was 5% vanillin sulfate solution.The plate was at heating on 105 ℃,when the spot was clear; the detection wavelength was 538 nm and the reference wavelength was at 700 nm. Result : The conduriol A was linear in range of 1.025~6.150 μg; the average recovery was 98.84%, RSD=2.02%(n=6).The average content of conduriol A was 2.08% in was simple, sensitive, accurate and had good reproducibility. Conclusion : The method can be used the identification and determination method of Guangxi Gymnema Sylvestre (Retz.)Schult and qualitatively.