黄连解毒汤的抗氧化作用及抑制乙酰胆碱酯酶活性的研究

Antioxidant Activity and Inhibition on Acetylcholinesterase by Huanglianjiedu Decoction

摘要:

目的 :筛选黄连解毒汤中与治疗老年性疾病有关的有效成分。 方法: 运用紫外分光光度法,建立体外抗氧化模型和抑制乙酰胆碱酯酶(AchE)活性实验模型,分析比较了黄连解毒汤全方、单味药及其主要成分的抗氧化作用和对乙酰胆碱酯酶的抑制作用。 结果: 复方黄连解毒汤的抗氧化活性比黄连、黄柏和栀子单味药强;黄芩及其主要成分黄芩苷(IC₅₀=12.06 μg ·mL^-1)具有较强的抗氧化活性;与对照品毒扁豆碱和加兰他敏比较,小檗碱(IC₅₀=9.52 μg ·mL^-1)对乙酰胆碱酯酶的抑制活性较高,其次是黄连(IC₅₀=18.89 μg ·mL^-1)水提物,而黄芩和栀子抑制活性不明显。 结论: 黄连解毒汤具有抗氧化和抑制乙酰胆碱酯酶活性作用,其中具有多羟基的黄酮类是抗氧化活性的主要成分;而生物碱类则是抑制乙酰胆碱酯酶的主要成分。

Abstract:

Objective: To investigate and screen the active compounds of Huanglianjiedu decoction(HLJD) for senile dementia. Method: Inhibition on acetylcholinesterase and antioxidant activity by HLJD was studied with ultraviolet spectrometry. The single herbs and the major compounds of HLJD were also determined and compared by a rapid and effective acetylcholinesterase inhibition assay and antioxidant assay. Result: The formula HLJD had a better antioxidant activity than single herbs except for Radix Scutellaria which is rich in polyphenolic compounds. The most active conpounds was baicalin (IC₅₀=12.06 μg ·mL-1). The results also indicate that the most active herbs and compounds for AChE inhibition were Coptidis Rhizoma (IC₅₀=18.89 μg ·mL^-1) and berberine (IC₅₀=9.52 μg ·mL^-1); Radix Scutellariae, Fructus Gardenia as well as the major compounds of them showed no or lower inhibitory activity. Conclusion: It can be concluded that alkaloid compounds in HLJD contribute to the inhibitory activity for acetylcholinesterase and flavanoids compounds contribute to the antioxidant activity.