板蓝根水溶性化学成分的研究

Studies on Water-soluble Chemical Constituent in Radix Isatidis

摘要:

目的: 提取、分离、鉴定板蓝根水溶性化学成分。 方法: 板蓝根水提取液上HP-20大孔吸附树脂,不同浓度乙醇梯度洗脱,洗脱部分经硅胶、凝胶、反相硅胶柱色谱反复分离、纯化,利用波谱技术确定化合物的结构。 结果: 从板蓝根水提取液中分离得到6个化合物,分别鉴定为(+)-异落叶松树脂醇(1)、落叶松树脂醇-4-O-β-D-吡喃型葡萄糖苷(2)、落叶松树脂醇-4,4'-二-O-β-D-吡喃型葡萄糖苷(3)、表告依春(4)、吲哚-3-乙腈-6-O-β-D-葡萄糖苷(5)、4-羟基-3-吲哚醛(6)。 结论: 化合物(6)为首次从该植物中分离得到。

Abstract:

Objective: To extract,separate and identify the water-soluble chemical constituent from Radix Isatidis. Method: The Radix Isatidis water extract underwent adsorption by HP-20 macroporous resin, the portion eluted by ethanol of different content was isolated and purified on silica gel column repeatedly. The structures of the compounds were identified by spectra data. Result: 6 compounds temporarily separated from Radix Isatidis water extract,were identified as: (+)-isolariciresinol(1) ,lariciresinol-4-bis-O-β-D-glucopyranoside(2 ),lariciresinol-4, 4'-bis-O-β-D-glucopyranoside(3 ),epigoitrin(4 ),indole-3-acetonitrile-6-O-β-D-glucopyranoside(5 ), 4-hydroxyindole-3-carboxaldehyde(6 ). Conclusion : For the first time, Compound 6 was isolated from Radix Isatidis.