虎杖HPLC指纹图谱研究与应用

Study on Chromatography Fingerprint of Polygonum Cuspidate by HPLC

摘要:

目的: 研究中药材虎杖的指纹图谱,并对虎杖苷、白藜芦醇及大黄素进行含量测定,为科学评价及有效控制虎杖质量提供了新的方法。 方法 : 利用反相高效液相色谱法分析了10个不同产地的虎杖样品,采用国家食品药品监督管理局推荐的"中药色谱指纹图谱相似度评价系统(2004A版)"计算处理,建立了由13个共有峰组成的虎杖指纹图谱,确定了虎杖苷、白藜芦醇及大黄素3个主要的指纹峰。 结果 : 试验方法样品处理简单,建立的虎杖指纹图谱稳定性、重复性好,可以用来区别不同产地虎杖药材的优劣,为控制虎杖的质量参考提供依据。

Abstract:

Objective: The chromatographic fingerprint of Polygonum Cuspidate was established and the content of polydatin, resveratrol and emodin was measured, which created a new method for evaluating and controlling the quality of Polygonum Cuspidate. Method :Using the Similarity Evaluation System of Chromatographic Fingerprint of Traditional Chinese Medicine (Version2004A) recommended by State Food & Drug Administration, 10 different original samples were analyzed and the chromatographic fingerprint of Polygonum cuspidate was constructed with 13 common fingerprint peaks, among which three main fingerprint peaks of polydatin, resveratrol and emodin were identified by reversed-phase high performance liquid chromatography (RP-HPLC). Result :With the simple sample treating technique, the established fingerprint of Polygonum Cuspidate was stable and repeatable, which could be used to distinguish the superior polygonum cuspidate from the inferior polygonum cuspidate from different places and provide an important tool for further quality control of Polygonum Cuspidate.