大黄中蒽醌类成分配伍前后的量变规律

Content Variation of Anthraquinones in Rhubarb Before and After Compatibilities

摘要:

目的 :目的:研究大黄分别与甘草、黄芩、赤芍、当归、黄连、木香、栀子配伍前后蒽醌类成分的含量变化。 方法: 以大黄素、大黄酸、芦荟大黄素、大黄酚、大黄素甲醚为指标,采用HPLC法测定大黄配伍前后游离和结合蒽醌类成分的含量。 结果 :配伍后游离蒽醌和结合蒽醌的总量有不同程度的降低,其中以与黄连配伍降低幅度最大;且大黄经配伍用药后,各蒽醌类成分含量均发生规律性变化。 结论 :大黄经配伍用药后,蒽醌含量发生变化,推测对降低其毒副作用有一定的贡献,该研究为大黄临床合理运用提供一定的依据。

Abstract:

Objective: To study content variation of anthraquinones in Rhubarb before and after compatibilities with liquorice root, baikal skullcap root, red peony root, Chinese angelica, Chinese goldthread rhizome, costusroot and gardenia. Method: An HPLC method was established to determine the contents of free and glycoside-combined anthraquinones namely aloe-emodin, rhein,emodin,chrysophanol and physcion in rhubarb before and after compatibility. Result: The total anthraquinones were decreased after compatibility, especially when it was compatible with Chinese goldthread rhizome. Besides, the content of various anthraquinones was changed regularly after compatibility. Conclusion: The content of the five different anthraquinones was influenced by different compatibilities, which may contribute to decrease the toxicity of rhubarb.The study provides the basis when rhubarb is used in clinic.