菩人丹超微粉对肥胖型2型糖尿病大鼠 糖代谢相关指标的影响

Effect of PRD Ultramicro-powder on the Indexes Correlated with Glycometabolism in Obese Type 2 Diabetes Mellitus Rats

摘要:

目的: 观察菩人丹超微粉(PRD supermicropowder)对2型糖尿病(Type 2 diabetes mellitus,T2DM)大鼠血糖、糖耐量、糖化血清蛋白、血清胰岛素及胰岛素敏感指数的影响,探讨菩人丹的降糖作用。 方法: 采用大鼠灌胃脂肪乳,辅以尾静脉快速iv小剂量链脲佐菌素,制备以胰岛素抵抗为特征的T2DM合并大血管病变的动物模型。成模大鼠随机分为菩人丹低、中、高治疗组,马来酸罗格列酮治疗组,模型组,各组分别ig给予相应药物治疗4周,测定用药前、用药后2周、4周大鼠随机血糖和治疗4周末空腹血清胰岛素、糖化血清蛋白含量,计算胰岛素敏感指数。 结果: 模型大鼠随机血糖和糖化血清蛋白含量较正常组上调,血清胰岛素、胰岛素敏感指数明显降低(P<0.01)。菩人丹低,中,高剂量及马来酸罗格列酮治疗组均能显著降低大鼠随机血糖和糖化血清蛋白含量(P<0.05~0.01),菩人丹中、高剂量组及马来酸罗格列酮组显著升高T2DM模型大鼠血清胰岛素含量(P<0.05~0.01),提高胰岛素敏感指数(P<0.05~0.01)。 结论: 菩人丹超微粉可降低T2DM大鼠过高的血糖和糖化血清蛋白含量,增加胰岛素敏感性,从而改善T2DM大鼠糖代谢。

Abstract:

Objective: To observe the influence of PRD(Pu Ren Dan) ultramicro-powder on the functional indexes correlated with glycometabolism in T2DM rats,including plasma glucose,glucose tolerance,glycosylated serum protein, serum insulin and insulin sensitivity index.And to investigate the hypoglycemic effect of PRD. Method: Wistar rats were fed fat milk,and small doses of streptozotocin was rapidly injected into tail vein.Animal models of type 2 diabetes with macroangiopathy characterized by insulin resistance were successfully prepared in a short time.The model rats were randomly divided into five groups including PRD low dose,PRD medium dose,PRD high dose,rosiglitazone maleate and model groups. Every group was respectively treated for 4 weeks with PRD low dose, PRD medium dose,PRD high dose,rosiglitazone maleate and water. Random plasma glucose of rats were respectively measured before treatment,after 2 weeks and 4 weeks with treatment. Fasting serum insulin and glycosylated serum protein content of rats were measured on the 4^th weekend.And Insulin sensitivity index were calculated. Result: As compared with normal group rats, random plasma glucose and glycosylated serum protein content of model group rats were increased,while serum insulin and insulin sensitivity index significantly were decreased(P<0.01). Random plasma glucose and glycosylated serum protein content of rats were all significantly reduced in PRD low, medium, high dose and rosiglitazone maleate groups (P<0.05~0.01).Serum insulin content and insulin sensitivity index of T2DM model rats treated by PRD medium, high dose and rosiglitazone maleate were significantly increased(P<0.05~0.01). Conclusion: Based on reducing the high level of plasma glucose and glycosylated serum protein content, PRD ultramicro-powder can enhance insulin sensitivity of T2DM rats and improve glycometabolism in T2DM rats.