中药胃肠舒对糖尿病大鼠胃窦平滑肌细胞线粒体的影响

Effect of Chinese Medicine Weichangshu on Mitochondrial in DiabeticMellitus Rats Stomach Smooth Mucle Cells

摘要:

目的 :研究中药胃肠舒对糖尿病(diabetes mellitus, DM)大鼠胃窦平滑肌细胞线粒体形态、结构、功能和蛋白的影响,探讨胃肠舒促胃肠运动的线粒体动能调控机制。 方法: 选择体重150-170g的标准SD大鼠随机分为4组:正常对照组、DM组、DM+胃肠舒Ⅰ组、DM+胃肠舒Ⅱ组。透射电子显微镜检测线粒体形态、结构的变化,流式细胞术检测线粒体跨膜电位的变化,荧光测定法检测线粒体呼吸链的变化,Western Blot检测线粒体蛋白的变化。 结果: 胃肠舒能改善DM大鼠胃窦平滑肌细胞线粒体的形态、结构,增强呼吸链功能,线粒体跨膜电位和ATP含量增高,细胞色素C氧化酶(cytochrome C oxidase, COX)活性增强,活化物氧自由基(ROS)释放减少,凋亡蛋白家族Bcl-2表达上调,Bax表达下调。 结论 :胃肠舒促胃肠运动可能与改善平滑肌的线粒体动能系统有关。

Abstract:

Objective: To study the mechanism of the chinese medicine Weichangshu on mitochondrial kinetic energy in diabetic mellitus rats stomach by measuring the changes of morphology, structure, function and protein of mitochondria. Method : Standard Sprague Dawley rats with a mean weight of 150-170g were randomly divided into 4 groups, including a control group, DM model group, DM+ WeichangshuⅠgroup, DM+ Weichangshu Ⅱ group. The mitochondrial morphology and structure were detected using transmission electron microscopy. The changes of mitochondrial transmembrane potential, respiratory chain and mitochondrial protein were detected using flow cytometry, fluorescence labeling and Western Blot respectively. Result :Weichangshu could ameliorate the mitochondrial morphology, structure and respiratory chain in diabetic mellitus rats stomach. Mitochondrial transmembrane potential, ATP level and activity of cytochrome C oxidase were increased. The release of ROS was decreased. The expression of Bcl-2 up-regulated and bax down-regulated. Conclusion :Weichangshu could promoted the gastrointestinal motility, which might be related with the regulation of mitochondrial kinetic energy.