花椒水提物给药大鼠尿液代谢组学研究

Metabonomics Study on the Urine in Rat Treated by Water Extract of Zanthoxylumschinifolium Siebet Zucc

摘要:

目的: 研究花椒水提物给药对大鼠尿样中内源性代谢产物的影响。 方法: 花椒水提物按0.342 8 g ·kg^-1给SD大鼠连续ig 30 d,收集尿样,通过Agilent1200 6410 三重四极杆质谱对尿液中内源性代谢物进行全扫描分析,SIMCA-P软件经过正交信号校正偏最小二乘法差别分析(OSC-PLS-DA)数据。 结果: 均值主成分分析结果显示花椒水提物给药15 d对尿样代谢产物的影响最大。与空白组相比,给药组大鼠尿样中cytidine,decanoylcarnitine,N-acetylglucosamine 6-phosphate等物质发生显著变化。 结论: 花椒水提物显著影响大鼠尿样中与抗肿瘤、镇痛、降血脂等药理作用相关物质的代谢,为进一步阐释花椒药性的研究工作提供了依据。

Abstract:

Objective: HPLC/MS/MS-based study on the changes of endogenous metabolites of Sprague-Dawley Rats administrated with water extract of Zanthoxylumschinifolium Siebet Zucc. Method: Ten Sprague-Dawley rats were successively administrated with Zanthoxylumschinifolium Siebet Zucc water extract (0.342 8 g ·kg^-1) for 30 days. Agilent1200 6410 Triple Quadrupole mass spectrometer was used for the analysis of endogenous metabolites in rat urine samples. These data were analyzed by OSC-PLS-DA. Result: The difference in metabolic profiles between the control group and the dosed group was well observed by OSC-PLS-DA analysis after administration of 15 days, 31 biomarkers were found, such as cytidine, decanoylcarnitine and N-acetylglucosamine 6-phosphate. Conclusion: The administration of Zanthoxylumschinifolium Siebet Zucc water extract could influence concentrations of endogenous matabolites related to anticancer, analgesic and hypolipidemic pharmacological actions. This work can provide basis for further researches on the interpretation of drug property of evodiae.