益智饮对阿尔茨海默病大鼠学习记忆能力的影响及抗氧化作用

Effect of Yizhiyin on Learning, Memory Ability and Antioxidation for Alzheimers Disease Rats Induced by Aβ1-42 in Rat

摘要:

目的: 观察益智饮对β-淀粉样肽(Aβ)_1-42致阿尔茨海默病(AD)模型大鼠学习记忆能力的影响及抗氧化作用。 方法: Aβ_1-42注射建立AD大鼠模型,分别给予不同剂量益智饮治疗。采用Morris水迷宫检测学习记忆能力、免疫组化检测大鼠脑组织Aβ蛋白表达情况;并测定脑组织超氧化物歧化酶(SOD) 含量、羟自由基抑制率。 结果: 与模型组相比,益智饮高、中、低剂量组的逃避潜伏期缩短(P<0.01);以高剂量组最优;高、中剂量组脑组织内Aβ聚集表达减少。益智饮各剂量组及阳性对照组大鼠脑组织SOD活性均高于模型组(P<0.01);高、中剂量组的羟自由基抑制率显著升高(P<0.01)。 结论: 益智饮能较好改善AD大鼠的学习记忆能力,减少脑内Aβ的聚集。其机制可能与通过升高SOD的活性和抑制羟自由基的生成有关。

Abstract:

Objective: To observe the effect of Yizhiyin on learning and memory ability in the rats with Alzheimers disease induced by Aβ_1-42 and the effect of antioxidation. Method: The rat model of AD were established by Aβ_1-42 injection into hippocampus, then those rats were treated by three different doses of Yizhiyin. Morris watermaze test was used for detecting learning and memory ability. Immunohistochemisty was used for detecting the expression of β-Amyloid, and the superoxide dismutase (SOD) and the hydroxy radical inhibition of brain were measured. Result: Compared with model group, the ability of learning and memory in high,middle and low doses group were improved (P<0.01).The results of immunohistochemisty showed that there were a lot of β-Amyloid deposition in the brain of model rats and a sprinkle expression in high and middle doses group. The activity of SOD was increased compared to those in model group in brain in each treatment group (P<0.01), the inhibition of hydroxy radical was increased (P<0.01) in each treatment group except the low dose group. Conclusion: The Yizhiyin could improve the learning and memory capability in AD rats efficiently, and decrease the β-Amyloid deposition. The mechanism of the Yizhiyin should be related to the increase in SOD and the inhibition of hydroxy radical.